The main applications of real-time quantitative PCR instrument real-time quantitative PCR:
Real-time quantitative PCR has a wide range of applications, including gene expression analysis based on relative quantitative analysis, absolute quantitative analysis of pathogens based on standard curve, qualitative SNP genotyping analysis of nucleic acid sequences after PCR amplification, and positive internal control-based positive control. / Negative result judgment
Includes the following features:
*Reaction plate setup guide, making experimental design very simple, even for complex multiplex quantitative experiments.
*Real-time monitoring of amplification reaction curve
* Automatically set the fluorescence baseline and automatically calculate the fluorescence threshold to greatly simplify the analysis of experimental results
*Absolute quantification of target genes using standard curve
*The software for relative quantification of gene expression provides a powerful result analysis display, which can automatically analyze the data of up to 10 96-well plate gene expression experiments at the same time, and automatically remove irrelevant data. This software is an option
*Automatic SNP typing software outputs typing results with intuitive graphs and automatically performs typing quality scoring
*Dissociation curve data collection is simple and easy to observe, and the dissociation curve data can be observed while the instrument is running
*It is very convenient to calibrate the corresponding sample well position when observing the real-time amplification curve
*Light source usage time monitoring and instrument system self-diagnosis program
Better excitation light source design greatly improves the detection sensitivity of long-wavelength red fluorescence
· Powerful and flexible reaction plate setup guide software makes reaction plate setup very convenient, and advanced experimental result display and automatic analysis tools make experimental data analysis and processing simpler and more intuitive
· The new semiconductor thermal cycling system supports 96-well plates and a single 0.2ml reaction tube.
· It can be upgraded to ultra-fast quantitative PCR reaction mode in the future, greatly shortening the quantitative PCR reaction time
· Small size, using laptop or desktop computer, suitable for any space laboratory.
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