The hot-start TAQ enzyme is modified. Generally, the active center will be exposed after heating at 95 degrees for 5-10 minutes, and polymerization will occur. This can effectively avoid template mismatch at low temperature, and avoid dimerization and non-specific amplification. Therefore, the specificity of the hot-start TAQ enzyme for amplification is very important

Products and Features Hot Start Tag DNA Polymerases are designed to enhance specificity, sensitivity and yield of DNA amplification. Using this enzyme, the reaction system can be prepared at room temperature, which is very convenient to use. This product is a recombinant hot-start Taq DNA polymerase based on chemical modification method, and a heat-labile blocking group is added to the amino acid residue of the protein molecule. The enzyme is inactive at room temperature, avoiding primer-dimer formation and non-specific extension. Thereby increasing the specificity of DNA amplification.

Heating at 95°C for 4 minutes restores enzyme activity. The activated enzyme has the same function as the hot-start Tag DNA polymerase: catalyzes the synthesis of DNA in the 5'→3' direction, has no detectable 3'→5' corrective exonuclease activity, and has a lower 5'→3' exonuclease Dicer activity. The enzyme also has deoxyribonuclease transfer activity, adding an additional adenine to the 3'-end of the PCR product. These activities were undetectable prior to activation.

The features of this product are:

1. High-yield amplification of complex templates.

2. Enzyme activity can be activated without heating at 95°C for 4 minutes.

3. High PCR specificity - reduce mismatch effect and reduce primer dimer generation.

4. Enhanced PCR sensitivity.

5. It is easy to use, and the PCR reaction system is prepared at room temperature.

6. The amplified product has a 3'-dA overhang. Specifications and ingredients Ingredients 100U packaging 500U packaging