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product description
This kit uses a silicon matrix material and buffer system that can bind DNA efficiently and specifically to recover DNA fragments from TAE or TBE agarose gels, while removing impurities such as proteins and inorganic salt ions. Fragments of 100bp-30kb size can be recovered, and the recovery rate can reach more than 80%. The DNA recovered using this kit can be applied to various routine operations, including enzyme digestion, PCR, sequencing, library screening, ligation and transformation experiments.
Product packaging
Kit composition | DP101-02 (100T) | DP101-03 (200T) | storage temperature |
Sol liquid | 100ml | 200m | RT |
rinse | 15ml×2 | 30ml×2 | |
eluent | 30ml | 60ml | |
adsorption column | 100 | 200 | |
collection tube | 100 | 200 |
Features
Fast : Simple operation, simple steps.
High efficiency : More than 80% of DNA can be recovered, and the recovery efficiency is high.
High purity : The recovered DNA is of high purity and can be used in subsequent experiments such as enzyme digestion, PCR, sequencing, ligation and transformation.
Diverse : single, double-stranded DNA and plasmid DNA can be recovered.
Storage Conditions
The kit can be stored for 12 months under dry conditions at room temperature (15-25°C). For long-term storage, it can be stored at 2-8 °C. If a precipitate occurs, it can be placed at room temperature for a period of time or in a 37 °C water bath for 10 minutes to dissolve the precipitate.
Experimental example
Comparison of nucleic acid recovery yields
Recovered fragment size (bp) | kit | Recovered nucleic acid concentration (ng/μl) | 260/280 | 260/230 |
150 | Company A | 65.2 | 1.881 | 1.782 |
SANSHIBIO | 67.3 | 1.971 | 1.962 | |
1000 | Company A | 87.6 | 1.913 | 2.832 |
SANSHIBIO | 90.185 _ | 1.986 | 3.53 | |
3000 | Company A | 54.9 3 | 1.880 | 1.051 |
SANSHIBIO | 49.4 | 1.882 | 2.380 |
Using the SANSHIBIO agarose gel recovery kit (DP101), the amplification products of different fragment sizes were recovered and purified by agarose gel electrophoresis, and the concentration was detected by Nanodrop. The experimental results show that the nucleic acid yield of DP101 is comparable to that of a similar product from a domestic commercial company A.
Location: Science and Technology Center, High-tech Zone, Shijiazhuang City, Hebei Province
Business Hotline: 186-3213-6937
Headquarters Tel: 0311-82970259
Email: sanshibio@126.com