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中山Taq DNA Polymerase (Mg2+ plus)

中山Taq DNA Polymerase (Mg2+ plus)

  • Category:中山Common PCR products
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  • Release time:2022-01-05 10:17:14
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产品介绍

product description

This product is Taq DNA Polymerase (Taq DNA Polymerase), referred to as Taq enzyme, is one of the most commonly used DNA polymerases. The PCR product amplified by this product has an A base at the 3' end and can be cloned into the T vector.

Product Specifications

Reagent composition
E001A-01(500U)
E001A-02(1000U)E001A-03(5000U)storage temperature
Taq DNA Polymerase
100μl200μl1000μl-20℃
10x PCR Buffer (Mg²﹢ plus)
1ml
1ml*2
1ml*10
dNTP Mixture (2.5mM each)
100μl200μl1000μl

Product Usage

PCR amplification of DNA

DNA sequencing

Features

Stable amplification easy to operate, stable amplification effect, and can achieve 1kb/30s extension speed.

Stable core performance: After accelerated test and room temperature storage verification, the core performance of this product is very stable.


Experimental example


             Example 1   Amplification effect
       Taq DNA Polymerase (E001A) was used to amplify fragments
with a length of 1kb, and the amplification results were analyzed by 1% agarose gel
electrophoresis . The amplification effect was comparable to
similar products of foreign company A. The three on the left are Taq DNA
polymerase , and the three on the right are E001A.



            Example 2   Enzyme Acceleration Experiment
       Taq DNA Polymerase (E001A) was
placed 10 days to amplify fragments with a length of 1kb, and
the amplification results were analyzed by 1% agarose gel electrophoresis. The amplification
effect was similar to that of foreign A The company's similar products are comparable, and the strips are bright. The three on the left
are Taq DNA polymerase of foreign company A, and the three on the right are
E001A 
.



   Example 3  Large fragments can be amplified without non-specific amplification
        . SANSHIBIO Taq DNA Polymerase (E001A
) was used to amplify fragments with a length of 3 kb, and the amplification results were analyzed by 1%
agarose gel electrophoresis. The experimental results show that the
amplification effect of SANSHIBIO Taq DNA Polymerase is equivalent to
the similar products of domestic company B and foreign company C, and there is no non
-specific amplification. The three on the left are E001A, the three in the middle are Taq DNA polymerase from domestic
company , and the three on the right are Taq
DNA polymerase from foreign company C.


产品参数

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