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product description
Taq DNA Polymerase, abbreviated as Taq enzyme, is one of the most commonly used DNA polymerases. It is expressed by E. coli cloned with Thermus aquaticus DNA Polymerase gene and purified by multiple steps. The PCR product amplified by this product has an A base at the 3' end and can be cloned into the T vector.
Product Specifications
Reagent composition | E001B-01(500U) | E001B-02(1000U) | E001B-03(5000U) | storage temperature |
Taq DNA Polymerase | 100μl | 200μl | 1000μl | -20℃ |
10x PCR Buffer (Mg2+ free) | 1ml | 1ml*2 | 1ml*10 | |
MgCl₂(25 mM) | 1ml | 1ml*2 | 1ml*10 | |
dNTP Mixture (2.5 mM each) | 100μl | 200μl | 1ml |
Product Usage
PCR amplification of DNA
DNA sequencing
Features
Stable amplification effect : easy to operate, stable amplification effect, and can achieve 1kb/30s extension speed .
Stable core performance: After accelerated experiments and room temperature storage verification, the core performance of this product is very stable.
Flexible system adjustment: Mg2+ concentration can be adjusted according to actual experimental needs.
Experimental example
Example 1 Amplification effect
Use Taq DNA Polymerase (E001B) to amplify the 1kb fragment,
The amplification results were analyzed by 1% agarose gel electrophoresis, and the amplification effect was comparable to the similar
products of foreign company A. The three on the left are Taq DNA polymerase from foreign company A, and the three on the right are E001B.
Example 2 Amplifies large fragments without non-specific amplification
SANSHIBIO Taq DNA Polymerase (E001B) was used to amplify the 3kb
fragment , and the amplification results were analyzed by 1% agarose gel electrophoresis. The experimental results show that the
amplification effect of SANSHIBIO Taq DNA Polymerase is equivalent to that of similar products of domestic company B, and there is
no non-specific amplification. The three on the left are E001B, and the three on the right are Taq DNA polymerase from foreign company A.
Location: Science and Technology Center, High-tech Zone, Shijiazhuang City, Hebei Province
Business Hotline: 186-3213-6937
Headquarters Tel: 0311-82970259
Email: sanshibio@126.com