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Working principle of automatic nucleic acid extraction instrument

2022-01-05 14:59:37
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Nucleic acid extractors are divided into two categories: one is large-scale automated, generally called an automatic liquid workstation; the other is a small automatic nucleic acid extractor, which uses packaged supporting reagents to automatically complete the extraction and purification process. Large-scale automatic liquid workstations have relatively high equipment costs and high operating costs, and are suitable for extracting thousands of samples of the same type at a time, so they are rarely used; while small automated automatic nucleic acid extraction instruments are easy to operate because of low equipment and operating costs. more and more applications.

The main function

Fully automatic purification of genomic DNA from blood, animal and plant tissues, dried blood slices, mouse tails and other samples; fully automatic purification of total viral nucleic acid from serum, plasma, swabs, nasopharyngeal secretions and other samples.

The automatic nucleic acid extraction instrument can be widely used in genomics, disease control medical treatment, food safety, forensic identification, blood transfusion safety, animal husbandry and molecular research, such as nasopharyngeal swabs, saliva, whole blood, serum, environmental samples, Nucleic acid extraction from various samples such as secretions, feces, animal tissues, plant tissues, bacteria, fungi, viruses, plasmids, etc., with stable results.

The fully automatic nucleic acid extraction instrument has the function of ultraviolet lamp disinfection to ensure that the operating space is free of pollution. Automatic operation, stable structure, high purification efficiency, UV disinfection lamp and multi-select temperature control design ensure that the experimental results are perfectly applied to researches such as disease control, blood transfusion safety, and food environment monitoring.

working principle:

1. In lysis buffer, cells are disrupted to release nucleic acids into the buffer;

2. Add magnetic beads to the buffer, and mix well with the specific coating material that adsorbs nucleic acids to the surface of the magnetic beads;

3. Wash the surface of the magnetic beads repeatedly to remove impurities such as unwanted nucleic acids, proteins or salts that were originally entrained;

4. Transfer the magnetic beads to the elution buffer and mix well. The nucleic acid is detached from the surface of the magnetic beads and dissolved in the elution buffer;

5. Remove the magnetic beads from the buffer and recover the nucleic acid solution.

Real-time fluorescence quantitative PCR instrument


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