The first-generation hot-start Taq enzyme is a DNA polymerase. The first generation refers to the natural polymerase directly extracted from Taq (which is a kind of bacteria) without chemical modification. In order to achieve better polymerization catalysis effect, it often needs to undergo enzyme engineering treatment, and the obtained polymerase catalyzes higher efficiency. Hot start refers to the reaction temperature conditions of this enzyme

Hot-start enzymes only start their enzymatic activity after a period of time at 95 degrees Celsius.

The temperature of common Taq DNA polymerase is 72°C.

The hot-start TAQ enzyme is modified. Generally, the active center will be exposed after heating at 95 degrees for 5-10 minutes, and polymerization will occur. This can effectively avoid template mismatch at low temperature, and avoid dimerization and non-specific amplification. Therefore, the specificity of hot-start TAQ enzyme for amplification is very important, but there are not many domestic companies that can produce hot-start TAQ enzyme with better performance.

Binuclear taq enzyme is a new type of taq enzyme developed on the basis of common taq enzyme. The dual-core dual-core powerful core can amplify PCR products faster and more efficiently.

The fidelity of the binuclear taq enzyme is much higher than that of the ordinary taq enzyme, and the amplification length is large. The human genome DNA template can well amplify the 8kb DNA fragment. It only needs 20%-50% of the enzyme amount of ordinary taq enzyme to obtain more amplification products than ordinary taq enzyme. The powerful polymerization ability requires only a few optimization steps to achieve a high success rate, and it is directly like ordinary taq enzymes. It can be used as taq enzyme, and does not need to do too much system adjustment.

The use of binuclear taq enzyme can effectively improve the yield of PCR.